EBNA2 induces IL-18Rβ expression. (A) (Left) RT-PCR for IL-18Rβ and the housekeeping gene, HPRT, in BL2, BL2-B95, BL2-P3, BL41, BL41-B95, and BL41-P3HR1 cells. (Right) Green fluorescence micrograph of BL41-B95 cells, reacted with a goat polyclonal antibody to IL-18Rβ, followed by biotinylated antibodies to goat IgG and FITC-coupled streptavidin. (B) RT-PCR for IL-18Rβ and HPRT in BL2, BL41, and BL41-P3 cells transfected with expression plasmids for EBNA2 or LMP1, as indicated. (C) RT-PCR for IL-18Rβ in EREB2-5 cells treated with estrogen (E) for the indicated times.