Figure 2.
Figure 2. EBNA2 but not LMP1, c-myc, or Notch induces IL-18Rα expression in nontransformed B cells. (A) Flow cytometric analysis of IL-18Rα expression (Figure 1B) in EREB2-5 cells treated with estrogen for the indicated times. (B) EREB2-5 cells were treated with estrogen (abbreviated E) and/or cycloheximide (CHX). Total RNA was prepared at the indicated time points, blotted, and probed with IL-18Rα cDNA. (C) Flow cytometric analysis of IL-18Rα expression in LMPtet-EREB cells treated with estrogen and/or tetracycline for 24 hours, as indicated. (D) Flow cytometric analysis of IL-18Rα expression in P493-6 cells treated with estrogen (EBNA2 on) and/or tetracycline (c-myc off) for 24 hours, as indicated. (E) Flow cytometric analysis of IL-18Rα expression in 1414 cells treated with estrogen (EBNA2 on) and/or tetracycline (Notch-IC off) for 24 or 96 hours, as indicated.

EBNA2 but not LMP1, c-myc, or Notch induces IL-18Rα expression in nontransformed B cells. (A) Flow cytometric analysis of IL-18Rα expression (Figure 1B) in EREB2-5 cells treated with estrogen for the indicated times. (B) EREB2-5 cells were treated with estrogen (abbreviated E) and/or cycloheximide (CHX). Total RNA was prepared at the indicated time points, blotted, and probed with IL-18Rα cDNA. (C) Flow cytometric analysis of IL-18Rα expression in LMPtet-EREB cells treated with estrogen and/or tetracycline for 24 hours, as indicated. (D) Flow cytometric analysis of IL-18Rα expression in P493-6 cells treated with estrogen (EBNA2 on) and/or tetracycline (c-myc off) for 24 hours, as indicated. (E) Flow cytometric analysis of IL-18Rα expression in 1414 cells treated with estrogen (EBNA2 on) and/or tetracycline (Notch-IC off) for 24 or 96 hours, as indicated.

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