Differential regulation of potential Smad7 target genes in primitive human hematopoietic cells. Smad7- and vector-transduced GFP⁺ cells expressing CD34 were isolated and used for RNA extraction and subsequent microarray analysis as detailed in “Materials and methods.” (A) A summarized subset of known Smads 1-9 and other genes involved in TGF-β/BMP pathway are shown, along with (B) genes previously associated with supporting either the myeloid or lymphoid lineage and (C) putative TGF-β target genes. Abbreviations: Smad ubiquitination regulatory factor (SMURF), activin receptor type 1 (ACVR1B), macrophage-stimulating protein 1 (MST1), interleukin (IL), connective tissue growth factor (Ctgf), latent TGF-β binding protein (Ltbp), tissue inhibitor of metalloproteinases 2 (TIMP2), ephrin-B1 (Efn B1), tenascin C (TNC).