Effect of CTLA-4-Fc on TTS mRNA expression in different cell types. (A) TTS mRNA expression was analyzed in isolated CD4⁺, CD8⁺, and CD19⁺ cells, and unseparated human PBMCs after 12 hours of culture in the absence or presence of CTLA-4-Fc. (B) Unseparated PBMCs were cultured in the absence or presence of CTLA-4-Fc for 12 hours. These PBMCs were then separated into CD4⁺, CD8⁺, and CD19⁺ cells and were analyzed for TTS mRNA expression. □ indicates control; ▪, CTLA-4-Fc. *P values less than .05 are based on 5 independent experiments. Mean values plus standard errors are shown.