Hemoglobin HPLC. Lysates from erythroid cell cultures from healthy individual N, simple Corfu heterozygote A07, compound Corfu heterozygote A09, and Corfu homozygote A06 were quantitated via cation-exchange HPLC. The peaks for HbA and HbF are indicated. The percentage of HbF from total hemoglobin, % HbF, is also indicated for each individual.