γ And β mRNA steady-state levels. Total RNA was isolated from 2 batches of erythroid cultures; α, γ, and β mRNA levels were quantitated via real-time PCR. γ And β mRNA levels are presented as percentage normalized to α-globin mRNA level for each individual. ▦ indicates γ mRNA; ▪, β mRNA. Percentage of γ mRNA from total β-like mRNA is also indicated as %γ, ie, 100% γ/(γ + β). (A) Normal individual N, simple Corfu heterozygote A07, compound Corfu heterozygote A09, and Corfu homozygote A06. (B) Normal individual N, compound Corfu heterozygotes A11 and A12, simple Corfu heterozygote A13, and compound heterozygote A10 (IVS-I-5/CD39).