RT-PCR analysis of γ gene transcription. Quantitative RT-PCR analysis of γ primary transcript levels in erythroid cell cultures from healthy individuals and patients with thalassemia. γ Primary transcript levels in patient versus normal cells were measured by quantitative RT-PCR with α levels as an endogenous reference. Results from 2 different sets of primary erythroid cultures are shown in panels A and B. The normal γ value is set to 1 in each experiment. (A) Relative γ transcript levels for a healthy individual N, simple Corfu heterozygote A07, and compound Corfu heterozygote A09. (B) Relative γ transcript levels for a healthy individual N, compound Corfu heterozygotes A11 and A12, simple Corfu heterozygote A13, and compound heterozygote A10 (IVS-I-5/CD39). Error bars indicate standard deviation between replicates.