Figure 2.
Figure 2. Ang1 and Ang2 induce neutrophil adhesion to HUVECs. Neutrophil adhesion to HUVECs was determined by cell surface adhesion assay under static conditions. PBS was used as a control. Due to slight variations in basal neutrophil adhesion between different experiments, we reported our data as relative neutrophil adhesion (%). Neutrophil recruitment induced by Ang1 (A) and Ang2 (B), both at 7.5 minutes, was determined at different concentrations (10–12 to 10–8 M; left panels) and in function of time at 10–9 M (5 to 15 minutes; right panels). Data are means ± SEM of at least 12 experiments; *P = .01 compared with PBS.

Ang1 and Ang2 induce neutrophil adhesion to HUVECs. Neutrophil adhesion to HUVECs was determined by cell surface adhesion assay under static conditions. PBS was used as a control. Due to slight variations in basal neutrophil adhesion between different experiments, we reported our data as relative neutrophil adhesion (%). Neutrophil recruitment induced by Ang1 (A) and Ang2 (B), both at 7.5 minutes, was determined at different concentrations (10–12 to 10–8 M; left panels) and in function of time at 10–9 M (5 to 15 minutes; right panels). Data are means ± SEM of at least 12 experiments; *P = .01 compared with PBS.

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