HLA-A2-dimer/peptide staining of precursor T cells and T cell being stimulated for 28 days of in vitro culture recognizing FMOD-derived peptides and the MAGE-3–derived peptide. A representative CLL patient (CLL-14) is shown. The precursor T-cell frequency of fibromodulin recognizing autologous T cells was tested without stimulation of the T cells. Then T cells were stimulated weekly for 28 days using autologous, native unpulsed CLL cells as APCs. The numbers given are the percentage of CD8-positive T cells costained with HLA-A2-dimer/peptide.