Figure 1.
Figure 1. All-trans-retinoic acid induces phosphorylation of the p70 S6 kinase. (A) NB-4 cells were treated with RA (1 μM) for the indicated times. Equal amounts of total cell lysates were analyzed by SDS-PAGE and immunoblotted with an antibody against the phosphorylated/activated form of the p70 S6 kinase on threonine 421 and serine 424. (B) The blot shown in panel A was stripped and reprobed with an anti-p70 S6 kinase antibody, to control for protein loading. (C) MCF-7 cells were treated with RA (1 μM) for the indicated times. Equal amounts of total cell lysates were analyzed by SDS-PAGE and immunoblotted with an antibody against the phosphorylated/activated form of the p70 S6 kinase on threonine 421 and serine 424. (D) The blot shown in panel C was stripped and reprobed with an anti-p70 S6 kinase antibody, to control for protein loading. (E) NB-4 cells were treated for 48 hours with the indicated doses of RA. Equal amounts of total cell lysates were analyzed by SDS-PAGE and immunoblotted with an antibody against the phosphorylated/activated form of the p70 S6 kinase on threonine 421 and serine 424. (F) The blot shown in panel E was stripped and reprobed with an anti-p70 S6 kinase antibody, to control for protein loading. (G) NB-4 cells were treated with RA for the indicated times. Equal amounts of total cell lysates were analyzed by SDS-PAGE and immunoblotted with an antibody against the phosphorylated/activated form of the p70 S6 kinase on threonine 421 and serine 424. (H) The blot shown in panel G was stripped and reprobed with an anti-p70 S6 kinase antibody, to control for protein loading. (I) RA-dependent induction of cell differentiation. NB-4 cells were incubated with RA for the indicated times in hours. The cells were subsequently stained with an anti-CD11b monoclonal antibody and analyzed by flow cytometry. (J) NB-4 cells were treated with RA for the indicated times. Equal amounts of total cell lysates were analyzed by SDS-PAGE and immunoblotted with an antibody against the phosphorylated/activated form of the p70 S6K. (K) The blot shown in panel J was stripped and reprobed with an anti-p70 S6K antibody.

All-trans-retinoic acid induces phosphorylation of the p70 S6 kinase. (A) NB-4 cells were treated with RA (1 μM) for the indicated times. Equal amounts of total cell lysates were analyzed by SDS-PAGE and immunoblotted with an antibody against the phosphorylated/activated form of the p70 S6 kinase on threonine 421 and serine 424. (B) The blot shown in panel A was stripped and reprobed with an anti-p70 S6 kinase antibody, to control for protein loading. (C) MCF-7 cells were treated with RA (1 μM) for the indicated times. Equal amounts of total cell lysates were analyzed by SDS-PAGE and immunoblotted with an antibody against the phosphorylated/activated form of the p70 S6 kinase on threonine 421 and serine 424. (D) The blot shown in panel C was stripped and reprobed with an anti-p70 S6 kinase antibody, to control for protein loading. (E) NB-4 cells were treated for 48 hours with the indicated doses of RA. Equal amounts of total cell lysates were analyzed by SDS-PAGE and immunoblotted with an antibody against the phosphorylated/activated form of the p70 S6 kinase on threonine 421 and serine 424. (F) The blot shown in panel E was stripped and reprobed with an anti-p70 S6 kinase antibody, to control for protein loading. (G) NB-4 cells were treated with RA for the indicated times. Equal amounts of total cell lysates were analyzed by SDS-PAGE and immunoblotted with an antibody against the phosphorylated/activated form of the p70 S6 kinase on threonine 421 and serine 424. (H) The blot shown in panel G was stripped and reprobed with an anti-p70 S6 kinase antibody, to control for protein loading. (I) RA-dependent induction of cell differentiation. NB-4 cells were incubated with RA for the indicated times in hours. The cells were subsequently stained with an anti-CD11b monoclonal antibody and analyzed by flow cytometry. (J) NB-4 cells were treated with RA for the indicated times. Equal amounts of total cell lysates were analyzed by SDS-PAGE and immunoblotted with an antibody against the phosphorylated/activated form of the p70 S6K. (K) The blot shown in panel J was stripped and reprobed with an anti-p70 S6K antibody.

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