Figure 3.
![Figure 3. Real-time RT-PCR analysis of gene expression repressed in response to hypoxia or AdCA5. Aliquots of the same RNA preparations used for microarray hybridization were analyzed by quantitative real-time RT-PCR. For each pair of experimental conditions (ie, 1% O2 vs 20% O2 [H] and AdCA5 vs AdLacZ [C]), the fold change in the levels of CCNE1, MPHOSP9, POLD, POLG, CDC2, PRC1, RRM2, and NEK4 mRNA was calculated. The mean and standard error for the 3 independent data sets are shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/105/2/10.1182_blood-2004-07-2958/6/zh80020572780003.jpeg?Expires=1724949335&Signature=Xmeb9xNi4Lf1EUwkuBHSdi3M7HWSvr45aqN2g4orRG7o32UoGcxy2SEYNiZjgvcOK9QTF5CJ-B42IC7utw8FzhDSFgQM~Uk8P~-~kYON6lWLvUrBFkQJ91ZhLSKFEosLhT0n3vRjBd7rAGRnBrSpbtSOyqamerDdKn~TqFVkktbb6moOrjp25nuwCFQgMbuj0MXURrDu2vF3DJ6Gze1nQ-7SLYMOA9TWCorP2~fzSmpOOzUcCgWkc7LxQ4CbIUbc8CnujxOJPPzKNWwqi3f4sh1c~jUWMP~zJQQWFY1xqvTRlXEj5jnaMIdoJ7fCOuqBv6N7uhmrmFuJPMIdiupQQg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Real-time RT-PCR analysis of gene expression repressed in response to hypoxia or AdCA5. Aliquots of the same RNA preparations used for microarray hybridization were analyzed by quantitative real-time RT-PCR. For each pair of experimental conditions (ie, 1% O2 vs 20% O2 [H] and AdCA5 vs AdLacZ [C]), the fold change in the levels of CCNE1, MPHOSP9, POLD, POLG, CDC2, PRC1, RRM2, and NEK4 mRNA was calculated. The mean and standard error for the 3 independent data sets are shown.
