Figure 2.
![Figure 2. Real-time RT-PCR analysis of gene expression induced in response to hypoxia or AdCA5. (A) Aliquots of the same 12 RNA preparations used for microarray hybridization (3 different EC primary cultures, 4 experimental conditions) were analyzed by quantitative real-time RT-PCR. For each pair of experimental conditions (ie, 1% O2 vs 20% O2 [H] and AdCA5 vs AdLacZ [C]) the fold induction of VEGF, PTGIS, and EDN1 mRNA expression was calculated (“Materials and methods”). The mean and standard error for the 3 independent data sets are shown. (B) ECs were exposed to hypoxia (1% O2) for 0 to 60 hours; total RNA was isolated; and the levels of CXCR4, EPOR, PTGIS, VEGF, and VEGFC mRNA were determined.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/105/2/10.1182_blood-2004-07-2958/6/zh80020572780002.jpeg?Expires=1724950053&Signature=VUs~pvdJxulB~GFwSnGluHob7dXm2zhNQWN6Zbcn44t2Wr36ZGyxedTMRgzkmDFB~I2TelMeQghAwW5NZgg7RvfKEt3eUW7xeohZz5j41dGQ5tQyU~KDFqPQLVjAeMKClbFtqP5AQw29GgoT3IJGhEn-qGdBz3~mgujH79plC0rZNazrsegzZAV9Ytei-xcML1C8qs25nbQ4d33iyfMBZi1Qc3CpeTlk5oSQUz-qaqU-u8A3zJiYAteG5AajZqIxn4P-h42TRY6mkAH-QFGYYzN2RD3~BdlZg6lJCwvHzIxMrwwDRM1XLS6bdgoX-DjY7pewdHFoq9jpIm49dRPcYQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Real-time RT-PCR analysis of gene expression induced in response to hypoxia or AdCA5. (A) Aliquots of the same 12 RNA preparations used for microarray hybridization (3 different EC primary cultures, 4 experimental conditions) were analyzed by quantitative real-time RT-PCR. For each pair of experimental conditions (ie, 1% O2 vs 20% O2 [H] and AdCA5 vs AdLacZ [C]) the fold induction of VEGF, PTGIS, and EDN1 mRNA expression was calculated (“Materials and methods”). The mean and standard error for the 3 independent data sets are shown. (B) ECs were exposed to hypoxia (1% O2) for 0 to 60 hours; total RNA was isolated; and the levels of CXCR4, EPOR, PTGIS, VEGF, and VEGFC mRNA were determined.
