Effects of leukotriene biosynthesis inhibitors on the expression of CD23, CD54, and CD150 in CD40L-activated B-CLL. Purified B-CLL cells were cultivated together with either L cells or CD40L-L cells in the absence or presence of MK-886 (100 nM), BWA4C (100 nM), and/or LTB4 (150 nM) for 96 hours. When inhibitors and/or LTB4 were used, B-CLL cells were pretreated with the compound(s) for 30 minutes in a serum-free medium prior to cultivation together with L cells or CD40L-L cells. B-CLL cells were collected and analyzed by FACS with antibodies against CD23, CD54, or CD150. The figure depicts 1 typical experiment out of 6. In order to more clearly demonstrate the different degree of expression of indicated antigen in the various samples, the inserted dotted line represents the expression of the indicated antigen in B-CLL cells stimulated with CD40L-L only. The shaded histograms show the expression of CD23 (left column), CD54 (middle column), or CD150 (right column). The open histograms represent the fluorescence of the cells stained with isotype-matched control mAbs of irrelevant specificity.
