Figure 1.
REHMA visualized. The top line illustrates the 271-bp sequence of the VκA27 light-chain V gene representing the signal peptide region, framework regions (FR1, FR2, FR3), and the complementarity determining regions (CDR1, CDR2). Fnu4HI sites, hot-spot, and TET- and FAM-fluorochrome-coupled primers (marked by an open and a black star, respectively) are indicated. Vertical black arrows indicate the sequenced cloned PCR product, codon 1-66, which is illustrated in Figure 4. The three bottom lines represent the 244-bp fragment of noncleaved hot-spot mutated VκA27 and the 106/109-bp fragments of cleaved hot-spot unmutated VκA27, respectively.

REHMA visualized. The top line illustrates the 271-bp sequence of the VκA27 light-chain V gene representing the signal peptide region, framework regions (FR1, FR2, FR3), and the complementarity determining regions (CDR1, CDR2). Fnu4HI sites, hot-spot, and TET- and FAM-fluorochrome-coupled primers (marked by an open and a black star, respectively) are indicated. Vertical black arrows indicate the sequenced cloned PCR product, codon 1-66, which is illustrated in Figure 4. The three bottom lines represent the 244-bp fragment of noncleaved hot-spot mutated VκA27 and the 106/109-bp fragments of cleaved hot-spot unmutated VκA27, respectively.

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