Figure 1.
Effect of THC on cell growth parameters. CEM, HEL-92, and HL60 cells were cultured continuously with THC (0-100 μM) for 2 days. (A) Cell viability was assessed by trypan blue dye analyses after 6 hours and 2 days, and the concentration required to cause a 50% reduction in cell viability (IC50) was calculated and is shown in the inset box. The effect of 2-day cultures with THC on cell cycle distribution was also assessed in the cell lines (B-D), and representative cell cycle histograms of cells cultured with 60 μM THC are shown (E). Each data point represents the means of at least 3 separate experiments, and SDs have been omitted for clarity.
