FACS analysis of C57BL/6 BMMΦs. (A) Density plot of the forward/side scatter analysis. Two subpopulations (R1, R3) are distinguishable and gated. (B) Histograms of the surface marker expression of region R1 BMMΦs. (C) Two-parameter dot plot analysis of BMMΦs of region R3. This region mostly consisted of various myeloid cells (not shown; see the second paragraph of “Multicolor FACS analysis of thioglycolate-elicited PECs and BMMΦ”), but also contained CD3⁺CD8⁺TCRβ⁺ T cells (∼7% of all living cells of region R3). The data are representative of 5 independent analyses.