IL-8 gene expression in CD34⁺ cells from patients with MMM and healthy subjects. Real-time quantitative PCR was performed with appropriate primers for IL-8 and GAPDH as described in “Materials and methods.” Amplification results were calculated by means of Sequence Detector Software 1.6.3. Mean IL-8/GAPDH normalized values with standard deviations are plotted for NPB (n = 3 pools of 6 to 7 blood samples) and NBM (n = 3). The IL-8/GAPDH ratio was individually calculated for the 3 patients with MMM.