American Society of Hematology

Figure 2.

$Expression of the TSLC1 gene in hematopoietic cells detected by RT-PCR. (A) Expression of the TSLC1 gene in cDNA from a series of hematopoietic cell fractions detected by RT-PCR. Each transcribed cDNA was amplified with the TSLC1-specific primer sets listed in “Patients, materials, and methods.” Each fraction of hematopoietic cells is indicated at the top of the lanes. Amplification of liver cDNA was used as a positive control (lane 10). (B) Expression of various genes in PHA-stimulated or unstimulated CD4+ T cells. Total RNA was prepared from CD4+ T cells stimulated with PHA or anti-CD3 and anti-CD28. cDNA was amplified using the primer sets for TSLC1, IL2RA, IL2, or β-actin as a quantitative control (described in “Patients, materials and, methods”). Control indicates unstimulated, +PHA, PHA-stimulated, and +αCD3/αCD28′, αCD3/αCD28′-stimulated CD4+ T cells.$

Expression of the TSLC1 gene in hematopoietic cells detected by RT-PCR. (A) Expression of the TSLC1 gene in cDNA from a series of hematopoietic cell fractions detected by RT-PCR. Each transcribed cDNA was amplified with the TSLC1-specific primer sets listed in “Patients, materials, and methods.” Each fraction of hematopoietic cells is indicated at the top of the lanes. Amplification of liver cDNA was used as a positive control (lane 10). (B) Expression of various genes in PHA-stimulated or unstimulated CD4⁺ T cells. Total RNA was prepared from CD4⁺ T cells stimulated with PHA or anti-CD3 and anti-CD28. cDNA was amplified using the primer sets for TSLC1, IL2RA, IL2, or β-actin as a quantitative control (described in “Patients, materials and, methods”). Control indicates unstimulated, +PHA, PHA-stimulated, and +αCD3/αCD28′, αCD3/αCD28′-stimulated CD4⁺ T cells.

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