Figure 2.
In vivo selection in dog G197 after multiple cycles of O6BG and temozolomide. (A-B) Flow cytometric analysis. (A) Percent EGFP-positive granulocytes (○) and lymphocytes (▪) from peripheral blood. (B) Percent EGFP-positive red blood cells (○) and platelets (▪) from peripheral blood. (C) Quantification of provirus in leukocytes from peripheral blood by Taqman-PCR. Arrows indicate the treatment cycles. O6BG was used at a fixed dose of 5 mg/kg with a maximal dose of 50 mg per administration. The doses of temozolomide were (1) 75 mg/m2, (2) 150 mg/m2, (3) 225 mg/m2, (4) 300 mg/m2, (5) 400 mg/m2, (6) 500 mg/m2, (7) 600 mg/m2, (8) 700 mg/m2, and (9) 800 mg/m2.
