Generation of angiostatin during wound healing. (A) The expression of plasminogen (arrow) and angiostatin forms (arrowheads) in normal cutaneous tissue (lane 1) as well as in a 5-day-old wound (lane 2) is shown. (B). The expression of plasminogen (arrow) and angiostatin forms (arrowheads) in wounds that were extracted at day 0 (lane 1), day 1 (lane 2), day 3 (lane 3), day 7 (lane 4), or day 10 (lane 5) after wound generation. Lane 6 indicates purified plasminogen (0.5 μg).