NF-κB signaling and MAP kinase activation in LPS-stimulated BMDCs from WT and IL-10^-/- mice. WT and IL-10^-/- BMDCs were stimulated with LPS (5 μg/mL) in the presence or absence of IL-10 (10 ng/mL); harvested at 0, 30, and 60 minutes; and Western blot analysis was performed for (A) phospho-IκB (P-IκB) and IκB, phospho-RelA and RelA, and (B) phospho-p38, p38, and phospho-JNK. The results are representative of 4 independent experiments. (C) WT and IL-10^-/- BMDCs were stimulated with LPS (5 μg/mL) for 0, 4, and 8 hours. ChIP analysis was performed as described in “Materials and methods.” Briefly, DNA was immunoprecipitated (IP) with cRel or RelA antibody, and PCR was performed with primers specific for the IL-12p40 gene promoter. Input samples show equal loading. Results shown are representative of 3 independent experiments.