IL-12p40 mRNA accumulation and protein secretion in LPS-stimulated BMDCs is NF-κB dependent. (A) IL-10^-/- BMDCs were stimulated with LPS (5 μg/mL) for 30 minutes, and immunofluorescence detecting cRel was performed as described in “Materials and methods.” (B) BMDCs from IL-10^-/- mice were infected with the IκB super repressor (Ad5IκBAA) or Ad5GFP (control) and subsequently stimulated with LPS (5 μg/mL). Cells were collected, RNA was isolated, and IL-12p40 mRNA was determined by RT-PCR. (C) IL-12p40 secretion by Ad5IκBAA- and Ad5GFP-infected IL-10^-/- BMDCs, stimulated by LPS (5 μg/mL) for 7 hours, as measured in triplicate supernatants by ELISA. All results are representative of 3 independent experiments. Error bars represent SEM; ^**P < .1.