Immunofluorescence results. (A) ATRX localizes to subnuclear PML bodies (in human fibroblasts). Reprinted with permission from Xue et al.76 (B) ATRX colocalizes with HP1β at heterochromatin (in murine L929 cells). Courtesy Drs D. Garrick and T.L. McDowell; used with permission. Images were captured with a Sensys CCD camera (Photometrics, Tucson, AZ) mounted on an Olympus BX51 microscope (Olympus) equipped with an Olympus UPlanApo 100 ×/1.35 oil immersion objective lens. Coverslips were mounted in Vectashield (Vector Laboratories, Peterborough, UK) containing 0.5 μg/mL DAPI. Images were acquired with MacProbe version 4.3 software (Applied Imaging, Newcastle, UK) and processed with Adobe Photoshop 7.0 (Adobe Systems).