Anchorage-independent growth of NIH 3T3 cells coexpressing NUP98-HOXA9 and cooperative genes. (A) Structure of retroviral constructs for NUP98-HOXA9 and cooperative genes. (B) Western blot analysis. Arrows indicate exogenously expressed proteins detected with the antibodies indicated at the bottom of each panel. (C) The numbers of colonies per 1 × 10⁴ cells seeded were determined 3 weeks after plating in soft agar. Values are the means with standard deviations of 3 experiments. Infected retroviral vectors are indicated at the bottom. Open bars and hatched bars indicate presence of the empty MSCV vector (-) or NUP98-HOXA9 retrovirus (+), respectively.