Replating analysis of BM cells derived from Tg mice. (A) Bone marrow was harvested from femurs of Tg mice or wild-type littermate controls, and 1 × 104 cells were plated in culture dishes containing methylcellulose and appropriate medium. Bulk cultures were harvested after 7 to 11 days in culture, and 1 × 104 cells were replated for each sample. Each point represents the number of colonies generated per 1 × 104 cells seeded. Cells (1 × 104) were plated in duplicate dishes, and the mean numbers of colonies for a representative experiment are shown. These data are representative of 5 similar experiments. (B) Classification of colonies in methylcellulose. Colonies at the second replating were analyzed morphologically. Granulocyte-macrophage colony-forming unit (CFU-GM), CFU of monocyte (CFU-mono), and CFU of mixed lineages (CFU-Mix) are increased in the transgenic bone marrow.
