Development and characteristics of leukemias in the Tg mice. (A) Leukemia-free survival curve of the Tg mice. (B) Wright-Giemsa-stained smear of peripheral blood showing leukemia cells displaying abnormal myelomonocytic morphology with immature nuclear segmentation at the myeloproliferative stage (magnification, × 1000). Images were visualized at indicated original magnification using an Olympus BX40 microscope equipped with a 40 ×/0.75 or a 100 ×/1.30 objective lens (Olympus, Tokyo, Japan). Images were photographed with an Olympus C4040 digital camera and acquired with Adobe Photoshop 4.0 J software (Adobe Systems, San Jose, CA). (C) Bone marrow smear of myeloid leukemia with maturation showing many myeloblasts (Wright-Geimsa; magnification, × 1000). (D) Histologic analysis showing leukemic blasts infiltrating in the liver (hematoxylin and eosin [H&E]; magnification, × 200). (E) Myeloid leukemia with maturation (H&E; magnification, × 400). (F) Myeloid leukemia without maturation (H&E; magnification, × 400). (G) Megakaryocytic leukemia (H&E; magnification, × 400). (H) Flow cytometric analysis of leukemic Tg spleen cells compared with wild-type littermates. Cells in suspension were stained with Gr-1, Mac-1, B220, and 2C11 antibodies conjugated with fluorescein isothiocyanate and depicted by the solid lines.