Generation of NUP98-HOXA9 transgenic mice. (A) The hCG-NUP98-HOXA9 transgenic construct. The NUP98-HOXA9 cDNA was inserted at the transcriptional start site of the hCG gene. The solid gray boxes represent the 5 exons of the hCG gene. The polyadenylation signal is provided by the hCG gene. The horizontal arrows indicate the location of RT-PCR primers. (B) RT-PCR analysis of bone marrow RNAs obtained from the transgenic mice of 5 lines (1, line 60; 2, line 131; 3, line 1514; 4, line 1583; 5, line 1589) and spleen of line 1589. Lines 131 and 1589 were used for further analysis. Two different kinds of transcripts were created due to alternative splicing of cathepsin G exons. β-actin was amplified from the same samples to check for RNA quality. mwm indicates 100 base pair (bp) ladder; wt, bone marrow of the wild-type mouse; and NC, negative control.