Migration to SDF-1α and CXCR expression of splenic T cells from control or Dok^–/– mice. Half a million purified T cells from Dok^–/– or control (Dok^+/+) mice were subjected to migration assay in a positive gradient of escalating doses of recombinant murine (rm) SDF-1α and a negative gradient and zero gradient of 100ng/mL of SDF-1α. After 4-hour migration, cells were enumerated by flow cytometry. (A) Input cells and migrated cells were stained with anti-CD4 and anti-CD8 antibodies to determine the numbers of CD4 and CD8 cells migrated. Migration index of whole, CD4, and CD8 cells are shown. Data are expressed as mean ± SEM from 6 mice of each group for CD4 cells (2 experiments with 3 mice each) and 3 mice of each group for whole or CD8 migration (*P < .05 compared with control cells. (B) Isolated T cells were stained with anti-CXCR4 and anti-CD4 or anti-CD8 antibodies. (left) Result of 1 representative experiment. (right) Percentage of positive cells and mean channel fluorescence (MCF) for CXCR4. Data are mean ± SEM of 3 mice per group for 1 experiment. NS indicates not significant.