SDF-1α/CXCL12 induces RasGAP association with Pyk2 in Jurkat cells. RasGAP immunoprecipitates were analyzed by Western blotting with anti-Pyk2 or anti-RasGAP Ab. Shown are results of 1 of 3 representative experiments. IP controls for RasGAP and PyK2 are shown in the bottom two panels. The total amount of PyK2 did not change after treatment with SDF-1, similar to the results noted in Figure 3A.