American Society of Hematology

Figure 2.

$Figure 2. Proliferative advantage of CB-CD34+ cells expressing the W51 Flt3-ITD in stromal coculture and liquid cultures. CB-CD34+ cells were transduced with MIGR1 or W51 and evaluated in stromal MS-5 coculture in Gartner media. Cells were demipopulated weekly for counting (A) and FACS analyses (B). (A) Absolute expansion of nonsorted, nonadherent cells. (B) Percentage of EGFP+ cells in the nonadherent fraction. (C) Cytoprep of nonadherent cells from week-2 MS-5 coculture of W51-transduced cells reveals primitive myelomonocytic and erythroid blasts. (D) Clusters of erythoblasts from the same cocultures. (E) Comparative FACS analysis of CD14 expression in nonadherent, week-2 MS-5 coculture of W51+ and MIGR1 cells highlights a drastic reduction in CD14 expression in Flt3-ITD–expressing cell cultures.$

Proliferative advantage of CB-CD34⁺ cells expressing the W51 Flt3-ITD in stromal coculture and liquid cultures. CB-CD34⁺ cells were transduced with MIGR1 or W51 and evaluated in stromal MS-5 coculture in Gartner media. Cells were demipopulated weekly for counting (A) and FACS analyses (B). (A) Absolute expansion of nonsorted, nonadherent cells. (B) Percentage of EGFP⁺ cells in the nonadherent fraction. (C) Cytoprep of nonadherent cells from week-2 MS-5 coculture of W51-transduced cells reveals primitive myelomonocytic and erythroid blasts. (D) Clusters of erythoblasts from the same cocultures. (E) Comparative FACS analysis of CD14 expression in nonadherent, week-2 MS-5 coculture of W51⁺ and MIGR1 cells highlights a drastic reduction in CD14 expression in Flt3-ITD–expressing cell cultures.

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