The effect of rPEDF, plPEDF, and the various rPEDF mutants on PEDF neurotrophic activity. The cells (2.5 × 105 cells/mL) were incubated with rPEDF, plPEDF, or the various rPEDF mutants (all at 20 nM) in MEM supplemented with 2 mM l-glutamine, antibiotics, and 0.1% 5 mg/mL insulin, 5 mg/mL transferrin, 5 mg/mL selenium (ITS). After 7 days in culture, the cells were transferred onto poly-d-lysine–coated plates, and their morphology and differentiation state were monitored by inverted microscope (Nikon TE 2000U connected to DUC camera) at various periods of time. The Y-79 morphology at 10 days after attachment is shown. (B) Quantitative analysis of the results presented in panel A is presented as a mean ± SD of 6 distinct experiments. Student t test was used to analyze statistical significance of the differences between cells treated with rPEDF and cells treated with the various PEDF forms (*P < .01; **P < .05). dia indicates diameter.
