Expression of hepcidin mRNA in isolated hepatocytes. Hepatocytes from either wild-type (+/+) or homozygous TfR2 Y245X mutant (m/m) mice were cultured for 48 hours in culture medium alone (control), DFO, iron-saturated Tf, IL-6, or LPS; their RNA was extracted and Northern blotted using murine hepcidin and β-actin probes.