Figure 1.
![Figure 1. Effects of CC-5013, rapamycin, and their combination on proliferation of MM cell lines and PBMCs. (A) Time- and dose-dependent inhibition of proliferation in sensitive MM cell lines (MM1.S, OPM2, SB645), demonstrated by measuring thymidine [3H-TdR] uptake during the last 8 hours of 48-hour cultures, in the presence of CC-5013 (μM), rapamycin (nM), or both in fixed-dose treatment. (B) Time- and dose-dependent inhibition of proliferation demonstrated by MTT in MM1.S cells. (C) Inhibition of DNA synthesis as measured by 3H-TdR uptake in resistant MM cell lines treated with CC-5013 and rapamycin. (D) Lack of toxicity of combined therapy on PBMCs, demonstrated by MTT assay. Error bars indicate one standard deviation.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/104/13/10.1182_blood-2004-06-2281/6/zh80240470780001.jpeg?Expires=1725198136&Signature=4Uy2eEbR9HItYpD4QOzzJAKK3lhe5yXh1AYVmYRSxiRF7Y2rWy-M51fuDvTh0scLI3pBm9wN6aLZN6JZVhkwMpm5WFrX18iKuvn7Sk5V2z5Ib6wck501Dtt73c0oYH9udD-ljPSSgb2PuLbAkjcrp8TvbDxhqcXLLP9sEk2jdjsPUViQ66jFGdTD7DRKE4S0VQZlycc5O6VwRUOdoitRwHNoIq9LEmVW-qQsvnsMdcTwhehP~mt61zCSekt528-zD5cnksf9GdZ5J2vJqTrAz50sLa0ZjZFPC7bScMPmRGsxQvO8VIUnd9AHtaTQuAXTsulpRRoFjD0e0KkhfolUQQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effects of CC-5013, rapamycin, and their combination on proliferation of MM cell lines and PBMCs. (A) Time- and dose-dependent inhibition of proliferation in sensitive MM cell lines (MM1.S, OPM2, SB645), demonstrated by measuring thymidine [3H-TdR] uptake during the last 8 hours of 48-hour cultures, in the presence of CC-5013 (μM), rapamycin (nM), or both in fixed-dose treatment. (B) Time- and dose-dependent inhibition of proliferation demonstrated by MTT in MM1.S cells. (C) Inhibition of DNA synthesis as measured by 3H-TdR uptake in resistant MM cell lines treated with CC-5013 and rapamycin. (D) Lack of toxicity of combined therapy on PBMCs, demonstrated by MTT assay. Error bars indicate one standard deviation.
