Time-dependent shifts in the volume distribution and in MCV and MCHC of SS discocytes during Ca²⁺-induced dehydration. (A) Volume distributions (V) during Ca²⁺-induced dehydration. Arrow indicates the time direction of displacement of the curves. Time intervals from the initial curve correspond to those shown for the MCV and MCHC points on the time-abscissa of the bottom panel. (B) MCVs (in femtoliters; •) and MCHCs (in g/dL; ○) are plotted as a function of time after A23187 addition to illustrate the inverse MCV-MCHC changes during Gardos-channel-mediated dehydration. (Inset) Change in SD of the volume distribution with time after A23187 addition. Notice the difference in the scale of the SD of MCV data compared with that in Figure 2B, indicating a much wider range of RBC volumes in this SS cell fraction than in the AA cells.