Migration patterns of the hemolysis curves of AA RBCs during Gardos-channel-mediated dehydration. (A) Patterns observed in 7 of 9 experiments with RBCs from 6 donors. Control curves (circles, in duplicate) show the distribution of osmotic fragilities in the RBC sample before Ca²⁺ permeabilization. For each curve, the sampling time is the interval after ionophore A23187 addition (set as t = 0), precisely when the 10-μL samples contained in the tips of a 12 × multipipette were discharged into the different hypotonic solutions. (B) Patterns observed in 2 of the 9 experiments in this series. Results in panels A and B were obtained with RBCs from blood drawn from the same donor on different dates.