Motheaten viable and SHIP-1^-/- mice exhibit a similar but more severe hematopoietic phenotype than Lyn^-/- mice. (A) FACS profiles of 6- to 8-week-old wild-type (+/+), Me^v/Me^v, and SHIP-1^-/- spleen and BM populations stained with CD71 and Ter119 mAbs. PI-positive and mature RBCs were excluded from the analysis. Relative percentages of immature erythroid (upper right quadrant) and more mature erythroid (lower right) cells are indicated for 4 mice of each genotype (± SD). (B) Total numbers of CD71⁺Ter119⁺ cells in the spleen and BM of +/+, Me^v/Me^v, SHIP-1^-/-, and Lyn^-/- mice are shown. Values represent the mean (± SD) of 5 C57BL/6 background mice per group, except Me^v/Mev, for which 2 mice per group are shown. (C) Mature erythroid (CFU-e) and immature erythroid (BFU-e) progenitors in +/+, Me^v/Me^v, and SHIP-1^-/- spleen and BM were determined at day 2 and day 10, respectively. Data represent the mean (± SEM) for 2 Me^v/Me^v mice and 4 +/+ and SHIP-1^-/- mice. (D) Myeloid progenitor content in the spleens of 8-week-old SHIP-1^-/- mice was assessed as in Figure 1 in the presence of the indicated cytokines. Values represent the mean (± SEM) for 4 animals in 2 experiments.