PML I recruits AML1b into nuclear bodies. (A) SaOS2 cells were transfected with LNCX-FLAG-AML1a, LNCX-FLAG-AML1b, or LNCX-HA-PML I. (B,C) AML1b, not AML1a, is recruited into NBs. SaOS2 cells were cotransfected with LNCX-FLAG-AML1a (B) or LNCX-FLAG-AML1b (C) together with LNCX-HA-PMLI. (D,E) Localization of AML1b and PML I mutants. SaOS2 cells were cotransfected with LNCX-FLAG-AML1b together with either LNCX-HA-PMLI d851 (D) or LNCX-HA-PMLI d592 (E). (F) AML1b was not colocalized with PML IV into NBs. SaOS2 cells were cotransfected with LNCX-FLAG-AML1b and LNCX-HA-PML IV. (G) AML1b is presented in the aggregates formed by PML I 3R. SaOS2 cells were cotransfected with LNCX-FLAG-AML1b and LNCX-HA-PML I 3R. In all experiments, AML1 was stained with anti-human AML rabbit polyclonal antibody, as shown by the green color, and PML was stained with anti-HA antibody (3F10), as shown by the orange color. Merging of the 2 colors results in a yellow signal, indicating the colocalization of 2 proteins. Nuclear staining with DAPI is shown by the blue color.