PML could interact with 2 other members of RUNX family AML2 and AML3. (A) BOSC23 cells were cotransfected with PML I and AMLs as indicated. The expression of AML proteins (upper) and PML I (middle) in the lysate of transfectants was analyzed by immunoblotting and detected with anti-AML antibody28 mixed with anti-AML3 antibody (Upstate Biotechnology, Charlottesville, VA) and anti-FLAG (M2) antibody, respectively. The lysate of transfectants were immunoprecipitated with anti-FLAG (M2) antibody, analyzed by immunoblotting, and probed with a mixture of anti-AML1 antibodies (lower). (B) Comparison of amino acid sequences for the C-terminal of AML1, AML2, and AML3. Identical amino acids are indicated in red. The boxed region corresponds to the PML I interacting domain of AML1.
