American Society of Hematology

Figure 5.

$Figure 5. Differential T- and B-cell trafficking in HEC-GlcNAc6ST-/- PLNs. (A) The ratio of homed T and B cells to WT and HEC-GlcNAc6ST lymphoid organs. One-hour homing of (B) T cells and (C) B cells to WT and HEC-GlcNAc6ST-/- lymphoid organs. n = 5 to 6 mice per group. (D-E) T-cell and (F-G) B-cell rolling fraction (D, F) and sticking efficiency (E, G) in WT and HEC-GlcNAc6ST-/- PLN venules. NS indicates not significant. *P < .05 and **P < .01 compared with WT. WT for T cells: n = 4 mice (order I, 2 venules; order II, 5; order III, 6; order IV, 5; order V, 5); for B cells: n = 4 mice (order I, 5 venules; order II, 8; order III, 12; order IV, 9; order V, 9 venules). HEC-GlcNAc6ST for T cells: n = 2 mice (order I, 2 venules; order II, 3; order III, 5; order IV, 7; order V, 5); for B cells: n = 3 mice (order I, n = 3 venules; order II, 5; order III, 10; order IV, 13; order V, 11).$

Differential T- and B-cell trafficking in HEC-GlcNAc6ST^-/- PLNs. (A) The ratio of homed T and B cells to WT and HEC-GlcNAc6ST lymphoid organs. One-hour homing of (B) T cells and (C) B cells to WT and HEC-GlcNAc6ST^-/- lymphoid organs. n = 5 to 6 mice per group. (D-E) T-cell and (F-G) B-cell rolling fraction (D, F) and sticking efficiency (E, G) in WT and HEC-GlcNAc6ST^-/- PLN venules. NS indicates not significant. ^*P < .05 and ^**P < .01 compared with WT. WT for T cells: n = 4 mice (order I, 2 venules; order II, 5; order III, 6; order IV, 5; order V, 5); for B cells: n = 4 mice (order I, 5 venules; order II, 8; order III, 12; order IV, 9; order V, 9 venules). HEC-GlcNAc6ST for T cells: n = 2 mice (order I, 2 venules; order II, 3; order III, 5; order IV, 7; order V, 5); for B cells: n = 3 mice (order I, n = 3 venules; order II, 5; order III, 10; order IV, 13; order V, 11).

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