American Society of Hematology

Figure 5.

$Figure 5. Poly I:C triggers IRF-3 activation in human NK cells. Polyclonal highly purified cultured NK cells (10 × 106 per sample) were stimulated with poly I:C (100 μg/mL) or PMA (50 ng/mL)/ionomycin (0.5 μM) (P/I) at 37°C for the indicated times. Cytosolic and nuclear extracts were separated on SDS-PAGE and immunoblotted with anti–IRF-3 Ab (top). Subcellular fractionation was checked by blotting with anti–β-tubulin (middle) and anti–USF-2 transcription factor (bottom) Abs. These data are representative of 1 out of 3 independent experiments.$

Poly I:C triggers IRF-3 activation in human NK cells. Polyclonal highly purified cultured NK cells (10 × 10⁶ per sample) were stimulated with poly I:C (100 μg/mL) or PMA (50 ng/mL)/ionomycin (0.5 μM) (P/I) at 37°C for the indicated times. Cytosolic and nuclear extracts were separated on SDS-PAGE and immunoblotted with anti–IRF-3 Ab (top). Subcellular fractionation was checked by blotting with anti–β-tubulin (middle) and anti–USF-2 transcription factor (bottom) Abs. These data are representative of 1 out of 3 independent experiments.

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