Dectin-1 traffics to lysosomes after zymosan binding and internalization. A time course of confocal images following the synchronous phagocytosis of zymosan in RAW 264.7 cells stably expressing HA-tagged Dectin-1 is shown. Cells were fixed, permeabilized, and stained to visualize Dectin-1 (green) and the late endosomal/lysosmal compartment marker LAMP-1 (red), using an APO oil immersion lens at 60× magnification, 1.4 NA. Zymosan phagosomes started becoming LAMP-1⁺ by 10 minutes and showed extensive colocalization of Dectin-1 and LAMP-1 by 30 minutes. The images are representative of 3 separate time-course experiments.