Figure 7.
![Transport of ADP in platelet membranes. (A-D) Platelet membrane vesicles (100 μg protein; crude membranes) were incubated with [3H]ADP (1 μM) in the presence of 0.4 mM ATP (▪) or 0.4 mM ATP + 1 mM orthovanadate (♦), and the vesicle-associated radioactivity was determined as described in “Patients, materials, and methods” (mean values ± SD, n = 3; 1 pmol × mg protein–1 = 1724 DPM). (A-B) Vesicles were preincubated for 45 minutes at 4°C in standard incubation buffer containing 250 mM sucrose (A) or in buffer containing 1 M sucrose (B). (C-D) ADP transport in the absence (C) or presence (D) of 100 μM dipyridamole.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/104/12/10.1182_blood-2003-12-4330/5/zh80230470130007.jpeg?Expires=1721907887&Signature=4cN57dDS0o4C2JOLVS~SitlQDJubEchtGmUxqdWrc63b~eq79L8UX8MSiZQxnq-peT-0qnAi3AEf0h6aSsQl0St5HhKJvzuG~ktwdJm7~4xVhD3rSo176iOzapgk8FfGlhUjbTMgSUC~KU46ML0rkpUiyQrxu0MR5lnC0b1tB1HrLKGOh13HoRO0NNlcS2VyFXVWC3R91s1wMvRJh73ExhRlJcXzRRn1RLRlsCxtCQy1rASo6hshp6cXwtmytsE9uz3DiEJgPsQFKdBnHcdQ~awUBlVTVdbMACyvrdrylb5uWBR7AzYVTXBNqzCxupe-zjo5WZqpz2YTsCMObiXR0g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Transport of ADP in platelet membranes. (A-D) Platelet membrane vesicles (100 μg protein; crude membranes) were incubated with [3H]ADP (1 μM) in the presence of 0.4 mM ATP (▪) or 0.4 mM ATP + 1 mM orthovanadate (♦), and the vesicle-associated radioactivity was determined as described in “Patients, materials, and methods” (mean values ± SD, n = 3; 1 pmol × mg protein–1 = 1724 DPM). (A-B) Vesicles were preincubated for 45 minutes at 4°C in standard incubation buffer containing 250 mM sucrose (A) or in buffer containing 1 M sucrose (B). (C-D) ADP transport in the absence (C) or presence (D) of 100 μM dipyridamole.
