Figure 5.
![Transport of cyclic nucleotides in platelet membranes. Platelet membrane vesicles (100 μg protein) were incubated with [3H]cGMP (left panel) or [3H]cAMP (right panel) (4 μM) in the presence of 4 mM ATP (▴) or 4 mM 5′-AMP (♦), and the vesicle-associated radioactivity was determined as described in “Patients, materials, and methods” (mean values ± SD, n = 3). The rate of net ATP-dependent transport (▪) was calculated by subtracting transport in the presence of 5′-AMP as a blank from transport in the presence of ATP. Right panel: ATP-dependent transport of cAMP in the presence (♦) or absence (▪) of 10 μM dipyridamole.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/104/12/10.1182_blood-2003-12-4330/5/zh80230470130005.jpeg?Expires=1721904978&Signature=M0uA06HeLBx5Q3SM76vXw6zi3vxg~Q1cfOWsLdjHAt3Y7gvOycmSZL~Bq3l~D0ouipGuWlqggfSWMaLfEnxP0CQkpIJV4HoKpRKmy0qs-hylA-PBjPIh1Aj9dlvkV0V0-nO62hcfslXQtD9Z2L-0qsefirULHxs2MdQQvjn4Mg3LMAaRnj~nW0lC5VzTHubNqawv58EzaJOEovdZnSPp1j7kqCM7Xp8yHrQzFR-EKQCOs16TPIZRCTP~g4cF-Qq7hEa5kkXhjZB4n9QuDzN5vvUMMHx~USsMNoU6q84E4O7YSwyeDX~sClcBj3aZj3dAc6dPbHpsgxV4pwvVVuWEgA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Transport of cyclic nucleotides in platelet membranes. Platelet membrane vesicles (100 μg protein) were incubated with [3H]cGMP (left panel) or [3H]cAMP (right panel) (4 μM) in the presence of 4 mM ATP (▴) or 4 mM 5′-AMP (♦), and the vesicle-associated radioactivity was determined as described in “Patients, materials, and methods” (mean values ± SD, n = 3). The rate of net ATP-dependent transport (▪) was calculated by subtracting transport in the presence of 5′-AMP as a blank from transport in the presence of ATP. Right panel: ATP-dependent transport of cAMP in the presence (♦) or absence (▪) of 10 μM dipyridamole.
