American Society of Hematology

Figure 3.

$Figure 3. Subcellular distribution of NF-κB in normal B cells. The main effectors of CD40 signaling are NF-κB transcription factors that translocate to the nucleus upon CD40 stimulation. Immunofluorescence was performed for the c-Rel NF-κB subunit on tonsillar sections to identify the cytoplasmic or nuclear localization of NF-κB in normal lymphoid tissues. The upper panel shows a tonsillar GC (magnification 10×). Dark zone (DZ), light zone (LZ), and the equivalent of the marginal zone in tonsil (“MZ”) are indicated. Green represents c-Rel; red, the nuclear counterstaining. The lower panels shows the 3 indicated GC regions at higher magnification (40 ×). In most GC B cells, NF-κB localizes in the cytoplasm: only a subset of CCs in the LZ shows nuclear staining for c-Rel (yellow arrows). Nuclear NF-κB is present in a fraction of cells in the tonsillar equivalent of the MZ that is populated mostly by memory B cells.$

Subcellular distribution of NF-κB in normal B cells. The main effectors of CD40 signaling are NF-κB transcription factors that translocate to the nucleus upon CD40 stimulation. Immunofluorescence was performed for the c-Rel NF-κB subunit on tonsillar sections to identify the cytoplasmic or nuclear localization of NF-κB in normal lymphoid tissues. The upper panel shows a tonsillar GC (magnification 10×). Dark zone (DZ), light zone (LZ), and the equivalent of the marginal zone in tonsil (“MZ”) are indicated. Green represents c-Rel; red, the nuclear counterstaining. The lower panels shows the 3 indicated GC regions at higher magnification (40 ×). In most GC B cells, NF-κB localizes in the cytoplasm: only a subset of CCs in the LZ shows nuclear staining for c-Rel (yellow arrows). Nuclear NF-κB is present in a fraction of cells in the tonsillar equivalent of the MZ that is populated mostly by memory B cells.

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