Characterization of intermediate CD14⁺CD1a⁺-proliferating cells during transdifferentiation. (A) Kinetic microscopic photographs of cultures during DC to OC transdifferentiation. Asterisks indicate OCs. (B) Analysis of cell proliferation during DC-to-OC transdifferentiation by kinetic FACS analyses performed on CFSE-labeled DCs cultured in the presence of M-CSF and RANKL. Numbers on scale bars indicate numbers of cell divisions (top row). Concomitant surface CD14 expression on dotted lines of isotypic controls (middle row). Dot plot shows simultaneous CD14 and CFSE fluorescence during transdifferentiation (bottom row). Negative control on CD14 axis is positioned by horizontal line. Results are representative of 3 independent experiments.