Figure 3.
![Figure 3. RFC gene expression and electrophoretic mobility shift assay in parental cells and their MTX-resistant sublines. Semiquantitative RT-PCR analysis of the RFC gene (A) along with a GAPDH control was performed as previously described.25,26,29 Electrophoretic mobility shift assay (B) was performed by the binding of nuclear proteins to their [32P]-labeled consensus double-stranded oligonucleotides followed by visualization with a Phosphorimager as detailed elsewhere.25,26](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/104/13/10.1182_blood-2004-04-1493/6/zh80240471130003.jpeg?Expires=1723281920&Signature=ndbROPv2Nuca7-J2nel5TsbM4mqCAKupuBIwyDUyvT6XfFZoGfdaxMX8-s9u07TAdWfP4RatS9CrV7dBS9VNrplADfkZy749nm5PD2FQV7hk5qtR8BbcWrA9Y4RMaN7h1YgPeILLUs3oXUxShGB8o9OFkQPIgGGUvzoz1hQo7iGNJovG1uFvgpCgxMBnoVOB1uEQncbf49ijuziWDf52Sbw41vgjhzuHn37iagV80~ep4WmPhqbwO~B1-efsN8mxviJkOI2M4zFzm7XOeEuaVe2yEwqTpIoz~8I8S4UQ-RLxe1qf2LJjSVMSHI9LbAgVNSj2~IgcJE4CcpkRNGIJFw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
RFC gene expression and electrophoretic mobility shift assay in parental cells and their MTX-resistant sublines. Semiquantitative RT-PCR analysis of the RFC gene (A) along with a GAPDH control was performed as previously described.25,26,29 Electrophoretic mobility shift assay (B) was performed by the binding of nuclear proteins to their [32P]-labeled consensus double-stranded oligonucleotides followed by visualization with a Phosphorimager as detailed elsewhere.25,26
