Colocalization of α_vβ₃ and FGFR1 using immunofluorescence. Confluent ECs (A) or HFFs (B) were treated with or without 100 ng/mL FGF-2 in the presence or absence of 10 μg/mL fibrinogen. After 1 hour, cells were washed and fixed with 3.7% formaldehyde and stained using 10 μg/mL FGFR1 and 7E3 antibody. FGFR is visualized as red fluorescence (i,iv,vii), α_vβ₃ is visualized as green fluorescence (ii,v,viii), and colocalization of FGF-2 and fibrinogen receptors is shown as yellow fluorescence (iii,vi,ix). Insets represent the background staining for red (i) and green (ii) fluorescence. Bars represent 25 μm.