Figure 2.
Effect of GRGDS peptide on EC proliferation. ECs were plated on gelatin-coated wells and allowed to adhere for 6 hours. The cells were then washed twice with McCoy medium and incubated in serum-free medium containing 1% Nutridoma and GRGDS at concentrations of 0.25 mM, 0.5 mM, or 1 mM. After 60 minutes, 25 ng/mL FGF-2, with or without 10 μg/mL fibrinogen, and 1 μCi/mL (0.037 MBq) 3H-thymidine was added to the medium. After 24 hours, cells were washed with PBS and isotope incorporated in DNA was precipitated with TCA, collected by vacuum filtration, and measured by scintillation counting. Results are the mean ± SD of 3 different experiments.
