American Society of Hematology

Figure 3.

EPCs are more resistant to exogenous oxidative stress. (A) HUVECs and (B) EPCs were incubated with LY-83583 for 18 hours, then stained with CM-H2DCF-DA and measured by FACS analysis. Representative histograms out of 4 independent experiments are shown. Data are mean ± SD, n = 4, *P < .05 versus control. (C) HUVECs and (D) EPCs were incubated with LY-83583 (10 μM) for 18 hours, stained with DHE, and measured by FACS analysis. Data are mean ± SD, n = 3. (E) HUVECs and (F) EPCs were incubated with H2O2 for 18 hours, stained with CM-H2DCF-DA, and measured by FACS analysis. Representative histograms out of 4 independent experiments are shown (upper panels). Data in lower panels are mean ± SD, n = 4, *P < .05 versus control. (G) HUVECs and (H) EPCs were incubated with the redox cycler LY-83583 (10 μM) or H2O2 (500 μM) for the indicated time points, stained with CM-H2DCF-DA, and measured by FACS analysis. Data are mean ± SD, n = 4.

EPCs are more resistant to exogenous oxidative stress. (A) HUVECs and (B) EPCs were incubated with LY-83583 for 18 hours, then stained with CM-H₂DCF-DA and measured by FACS analysis. Representative histograms out of 4 independent experiments are shown. Data are mean ± SD, n = 4, *P < .05 versus control. (C) HUVECs and (D) EPCs were incubated with LY-83583 (10 μM) for 18 hours, stained with DHE, and measured by FACS analysis. Data are mean ± SD, n = 3. (E) HUVECs and (F) EPCs were incubated with H₂O₂ for 18 hours, stained with CM-H₂DCF-DA, and measured by FACS analysis. Representative histograms out of 4 independent experiments are shown (upper panels). Data in lower panels are mean ± SD, n = 4, *P < .05 versus control. (G) HUVECs and (H) EPCs were incubated with the redox cycler LY-83583 (10 μM) or H₂O₂ (500 μM) for the indicated time points, stained with CM-H₂DCF-DA, and measured by FACS analysis. Data are mean ± SD, n = 4.

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