Leukocyte rolling flux and leukocyte adhesion in contact hypersensitivity (CHS) model 2 and 24 hours after challenge. (A,C) Leukocyte rolling flux at 2 and 24 hours after challenge, respectively. (B,D) Leukocyte adhesion at 2 and 24 hours after challenge, respectively. The effects of anti–P- and E-selectin antibodies in combination are shown as well as the anti–PSGL-1 antibody (4RA10) and in PSGL-1^-/- mice. Finally, the effect of anti–E-selectin antibody in PSGL-1^-/- is shown. (E,F) Flow cytometric analysis showing the binding in vitro of recombinant mouse E-selectin chimera to neutrophils from C57Bl/6 at 2 and 24 hours of CHS, respectively. Data are presented as mean ± SEM. ^*P < .05 relative to untreated value.